Phytosterol Profile in Iraqi Lactuca serriola after Purification and Isolation by Combiflash and HPLC (Conference Paper )#

One of these plants utilized in traditional medicine is Lactuca seriolla Linn., which belongs to the Asteraceae family. It goes by a variety common names world, including prickly lettuce, wild jagged and Kahu khas. The work aimed isolate characterize some bioactive constituent(s) from aerial part serriola utilizing Combiflash NEXTGEN high-performance liquid chromatography (HPLC). (aerial part) was extracted with 80% ethanol, then fractionated hexane. Then 250 mg hexane extract mixed 4 g silica gel loaded cartilage, bounded gold column combi flash using solvent system comprised ethyl acetate: n-hexane (10% acetate 90% v/v) elute column. fractions resulting were analyzed thin layer white needle-like crystalline substances isolated via chromatography. product β-sitosterol (11 mg) stigmasterol (7 obtained fraction.